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BBS1 Polyclonal antibody

BBS1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 21118-1-AP

Synonyms

Bardet Biedl syndrome 1, BBS1, BBS2 like protein 2, BBS2L2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using BBS1 Polyclonal antibody (21118-1-AP)

    WB analysis of mouse kidney using 21118-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21118-1-AP (BBS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using BBS1 Polyclonal antibody (21118-1-AP)

    WB analysis of mouse brain using 21118-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21118-1-AP (BBS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using BBS1 Polyclonal antibody (21118-1-AP)

    WB analysis of mouse heart using 21118-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21118-1-AP (BBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using BBS1 Polyclonal antibody (21118-1-AP)

    IP experiment of mouse brain using 21118-1-AP

    IP result of anti-BBS1 (IP:21118-1-AP, 4ug; Detection:21118-1-AP 1:600) with mouse brain tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using BBS1 Polyclonal antibody (21118-1-AP)

    IF Staining of hTERT-RPE1 cells using 21118-1-AP

    IF result (base of ciliary stain) of anti-BBS1 (21118-1-AP, 1:50) with with serum-starved hTERT-RPE1 cells by Dr. Moshe Kim.

Tested Applications

Positive WB detected inmouse kidney tissue, mouse brain tissue, mouse heart tissue
Positive IP detected inmouse brain tissue
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21118-1-AP is BBS1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS1 fusion protein Ag15460
Full Name Bardet-Biedl syndrome 1
Calculated Molecular Weight 593 aa, 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC047642
Gene Symbol BBS1
Gene ID (NCBI) 582
RRIDAB_2878814
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BBS1 antibody 21118-1-APDownload protocol
IP protocol for BBS1 antibody 21118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO J

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Authors - Shichao Duan
View Article
humanIF

J Biol Chem

Non-redundant roles of DIAPHs in primary ciliogenesis.

Authors - Oliva Palander
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Jerome (Verified Customer) (05-07-2019)

Nuclear staining, and perinuclear staining that looks like a Golgi

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 100
  • Cell Tissue Type: Hela Cells
BBS1 Antibody Immunofluorescence, validation (100 dilution) in Hela Cells (Cat no:21118-1-AP)