BBS2 Polyclonal antibody

BBS2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11188-2-AP

Synonyms

Bardet Biedl syndrome 2, BBS, BBS2



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11188-2-AP targets BBS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS2 fusion protein Ag1662
Full Name Bardet-Biedl syndrome 2
Calculated molecular weight 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC014140
Gene symbol BBS2
Gene ID (NCBI) 583
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BBS2 is one of seven BBS proteins that form the stable core of a protein complex (BBSome complex, 438kd) which is required for ciliogenesis but is dispensable for centriolar satellite function.

Protocols

Product Specific Protocols
WB protocol for BBS2 antibody 11188-2-APDownload protocol
IP protocol for BBS2 antibody 11188-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Genet Genomic Med

Refining genotype-phenotype correlation in Alström syndrome through study of primary human fibroblasts.

Authors - Jian-Hua Chen
mouseWB

Invest Ophthalmol Vis Sci

Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis.

Authors - Satyabrata Sinha
human,mouse,ratIHC,IF

J Comp Neurol

Patterns of expression of Bardet-Biedl syndrome proteins in the mammalian cochlea suggest noncentrosomal functions.

Authors - May-Simera Helen L HL
humanIF

Mol Biol Cell

Single-particle tracking localization microscopy reveals nonaxonemal dynamics of intraflagellar transport proteins at the base of mammalian primary cilia.

Authors - T Tony Yang
mouseWB

Dev Neurobiol

A truncating mutation of Alms1 reduces the number of hypothalamic neuronal cilia in obese mice.

Authors - Heydet Déborah D
humanIF

Sci Rep

Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells.

Authors - Luciane M Silva

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Deng (Verified Customer) (07-30-2019)

This antibody stained primary cilia in cultured IMCD3 cells.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1;250
  • Cell Tissue Type: IMCD3 cells
BBS2 Antibody Immunofluorescence, validation (1;250 dilution) in IMCD3 cells (Cat no:11188-2-AP)