|Positive WB detected in||mouse testis tissue|
|Positive IP detected in||mouse testis tissue|
|Positive IF detected in||hTERT-RPE1 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11188-2-AP targets BBS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BBS2 fusion protein Ag1662|
|Full Name||Bardet-Biedl syndrome 2|
|Calculated molecular weight||80 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC014140|
|Gene ID (NCBI)||583|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BBS2 is one of seven BBS proteins that form the stable core of a protein complex (BBSome complex, 438kd) which is required for ciliogenesis but is dispensable for centriolar satellite function.
Mol Genet Genomic Med
Refining genotype-phenotype correlation in Alström syndrome through study of primary human fibroblasts.
Invest Ophthalmol Vis Sci
Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis.
J Comp Neurol
Patterns of expression of Bardet-Biedl syndrome proteins in the mammalian cochlea suggest noncentrosomal functions.
Mol Biol Cell
Single-particle tracking localization microscopy reveals nonaxonemal dynamics of intraflagellar transport proteins at the base of mammalian primary cilia.
A truncating mutation of Alms1 reduces the number of hypothalamic neuronal cilia in obese mice.
Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Deng (Verified Customer) (07-30-2019)
This antibody stained primary cilia in cultured IMCD3 cells.