Tested Applications
| Positive WB detected in | HEK-293 cells, mouse kidney tissue |
| Positive IHC detected in | mouse stomach tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12766-1-AP targets BBS4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3430 Product name: Recombinant human BBS4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC027624 Sequence: MAEERVATRTQFPVSTESQKPRQKKAPEFPILEKQNWLIHLHYIRKDYEACKAVIKEQLQETQGLCEYAIYVQALIFRLEGNIQESLELFQTCAVLSPQSADNLKQVARSLFLLGKHKAAIEVYNEAAKLNQKDWEISHNLGVCYIYLKQFNKAQDQLHNALNLNRHDLTYIMLGKIHLLEGDLDKAIEVYKKAVEFSPENTELLTTLGLLYLQLGIYQKAFEHLGNALTYDPTNYKAILAAGSMMQTHGDFDVALTKYRVVACAVPESPPLWNNIGMCFFGKKKYVAAISCLKRANYLAPFDWKILYNLGLVHLTMQQYASAFHFLSAAINFQPKMGELYMLLAVALTNL Predict reactive species |
| Full Name | Bardet-Biedl syndrome 4 |
| Calculated Molecular Weight | 519 aa, 58 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC027624 |
| Gene Symbol | BBS4 |
| Gene ID (NCBI) | 585 |
| RRID | AB_10596774 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BBS4 antibody 12766-1-AP | Download protocol |
| IHC protocol for BBS4 antibody 12766-1-AP | Download protocol |
| WB protocol for BBS4 antibody 12766-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody (catalog no. 12766-1-AP) has 23 citations across journals including Science, Nature Communications, Autophagy, Developmental Cell, Cell Reports, PLoS Biology, EMBO Reports, Journal of Cell Biology, Nucleic Acids Research, Scientific Reports, eLife, and others. Research fields span ciliogenesis, centriolar satellites, Bardet-Biedl syndrome, intraflagellar transport, centrosome biology, cell cycle regulation, and autophagy.
| Species | Application | Title |
|---|---|---|
Autophagy The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts. | ||
Nat Commun Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets. | ||
Nat Commun An actin filament branching surveillance system regulates cell cycle progression, cytokinesis and primary ciliogenesis | ||
Nucleic Acids Res Programmable RNA 5-methylcytosine (m5C) modification of cellular RNAs by dCasRx conjugated methyltransferase and demethylase | ||
Reviews
What customers say
One reviewer used this antibody at a 1:1,000 dilution for Western Blot on HEK293T cells and rated it 4 out of 5 stars. They detected the expected band above 47 kDa (consistent with BBS4's expected size of 50–60 kDa), but also observed a much stronger non-specific band just below the 20 kDa marker, suggesting some background or non-specific binding at that lower molecular weight.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christine (Verified Customer) (01-27-2023) | Detect 1 band above 47 kDa marker (expected size of BBS4 is 50-60 kDa), but also a much stronger band just below the 20 kDa marker
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