Brevican Polyclonal antibody

Brevican Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19017-1-AP

Synonyms

BCAN, BEHAB, Brevican, Brevican core protein, CSPG7



Tested Applications

Positive WB detected inhuman brain tissue, COLO 320 cells, SH-SY5Y cells, Y79 cells
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19017-1-AP is Brevican Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Brevican fusion protein Ag13552
Full Name brevican
Calculated molecular weight 99 kDa
Observed molecular weight 145 kDa, 80 kDa
GenBank accession numberBC010571
Gene symbol BCAN
Gene ID (NCBI) 63827
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Brevican is a brain-specific proteoglycan belonging to the aggrecan family that share a number of structural features. Brevican contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Expression of brevican is highly specific in the brain and increases as the brain develops. It may play a role in maintaining the extracellular environment of mature brain as a major constituent of the adult brain extracellular matrix (PMID:9117262). Brevican core protein exists as a 145 kDa full-length form and a 80 kDa N terminally truncated form (PMID: 8144512).

Protocols

Product Specific Protocols
WB protocol for Brevican antibody 19017-1-APDownload protocol
IHC protocol for Brevican antibody 19017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols