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  • Featured Product
  • KD/KO Validated

BCL10 Polyclonal antibody

BCL10 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 17732-1-AP

Synonyms

B cell CLL/lymphoma 10, B cell lymphoma/leukemia 10, BCL10, c E10, CARD like apoptotic protein, CARMEN, cCARMEN, Cellular E10, Cellular homolog of vCARMEN, CIPER, CLAP, hCLAP, mE10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCL10 Polyclonal antibody (17732-1-AP)

    WB analysis using 17732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17732-1-AP (BCL10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using BCL10 Polyclonal antibody (17732-1-AP)

    WB analysis of K-562 using 17732-1-AP

    WB result of BCL10 antibody (17732-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL10 transfected K-562 cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BCL10 Polyclonal antibody (17732-1-AP)

    IHC staining of human breast cancer using 17732-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BCL10 Polyclonal antibody (17732-1-AP)

    IHC staining of human breast cancer using 17732-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL10 Polyclonal antibody (17732-1-AP)

    IHC staining of human lymphoma using 17732-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL10 Polyclonal antibody (17732-1-AP)

    IHC staining of human lymphoma using 17732-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Jurkat cells using BCL10 Polyclonal antibody (17732-1-AP)

    FC experiment of Jurkat using 17732-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human BCL10 (17732-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17732-1-AP targets BCL10 in WB, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL10 fusion protein Ag12170
Full Name B-cell CLL/lymphoma 10
Calculated Molecular Weight 233 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC053617
Gene Symbol BCL10
Gene ID (NCBI) 8915
RRIDAB_2064860
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.

Protocols

Product Specific Protocols
WB protocol for BCL10 antibody 17732-1-APDownload protocol
IHC protocol for BCL10 antibody 17732-1-APDownload protocol
FC protocol for BCL10 antibody 17732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols