Bestrophin-1 Monoclonal antibody

Bestrophin-1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4D5F1

Cat no : 60326-1-Ig

Synonyms

ARB, BEST, BEST1, bestrophin 1, Bestrophin-1, BMD, TU15B, VMD2



Tested Applications

Positive WB detected inY79 cells, Transfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

60326-1-Ig targets Bestrophin-1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Bestrophin-1 fusion protein Ag15129
Full Name bestrophin 1
Calculated molecular weight 585 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC015220
Gene symbol BEST1
Gene ID (NCBI) 7439
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bestrophin-1 (BEST1) is a 68 kDa transmembrane protein which belongs to the bestrophin family of anion channels. It is predominantly expressed in the basolateral membrane of the retinal pigment epithelium. Studies show that bestrophin 1 functions as a Ca(2+)-dependent Cl(-) channel and modulator of voltage-dependent L-type Ca(2+) channels. It may also contribute to the basolateral cell conductance in airway epithelial cells. This protein is encoded by the VMD2 gene. Mutations in VMD2 can lead to different types of retinal or macular degenerations, including Best vitelliform macular dystrophy (BMD), autosomal recessive bestrophinopathy (ARB), autosomal dominant vitreoretinochoroidopathy (ADVIRC) and adult-onset vitelliform macular dystrophy (AVMD).

Protocols

Product Specific Protocols
WB protocol for Bestrophin-1 antibody 60326-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols