Bestrophin-3 Polyclonal antibody

Bestrophin-3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20443-1-AP

Synonyms

BEST3, Bestrophin 3, Bestrophin-3, VMD2L3



Tested Applications

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20443-1-AP is Bestrophin-3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bestrophin-3 fusion protein Ag14255
Full Name bestrophin 3
Calculated molecular weight 668 aa, 76 kDa
Observed molecular weight 76-78 kDa, 50-55 kDa
GenBank accession numberBC028087
Gene symbol BEST3
Gene ID (NCBI) 144453
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bestrophin-3 (BEST3) is a member of the Bestrophin (VMD2) Ca2+-activated Cl channel gene family. Bestrophin-3 is involved in the regulation of cell proliferation, apoptosis and differentiation of a variety of physiological functions (PMID: 25329324). Human BEST3 gene is located at chromosome 12q15. The expression of BEST3 was predominantly observed in skeletal muscle and weaker in brain, spinal cord, bone marrow, retina, thymus, and testis (PMID: 12032738).

Protocols

Product Specific Protocols
WB protocol for Bestrophin-3 antibody 20443-1-APDownload protocol
IHC protocol for Bestrophin-3 antibody 20443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Rep

A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum.

Authors - Lichang Wu