BMP2 Monoclonal antibody

BMP2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1C5C2

Cat no : 66383-1-Ig

Synonyms

BMP 2, BMP 2A, BMP2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A



Tested Applications

Positive WB detected inpig bone marrow tissue, Caco-2 cells, HeLa cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66383-1-Ig targets BMP2 in WB, IHC,ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BMP2 fusion protein Ag13501
Full Name bone morphogenetic protein 2
Calculated molecular weight 396 aa, 44 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC069214
Gene symbol BMP2
Gene ID (NCBI) 650
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction.

Protocols

Product Specific Protocols
WB protocol for BMP2 antibody 66383-1-IgDownload protocol
IHC protocol for BMP2 antibody 66383-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IHC

J Bone Miner Metab

MnTBAP inhibits bone loss in ovariectomized rats by reducing mitochondrial oxidative stress in osteoblasts.

Authors - Xiangchang Cao
humanWB

Endocrinology

PMID: 31748790

Authors - Alison M Neff
humanWB

Angiogenesis

Linalool inhibits the angiogenic activity of endothelial cells by downregulating intracellular ATP levels and activating TRPM8.

Authors - Vivien Becker