Tested Applications
| Positive WB detected in | pig bone marrow tissue, Caco-2 cells, HeLa cells, pig aorta tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66383-1-Ig targets BMP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, pig, rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13501 Product name: Recombinant human BMP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-396 aa of BC069214 Sequence: MVAGTRCLLALLLPQVLLGGAAGLVPELGRRKFAAASSGRPSSQPSDEVLSEFELRLLSMFGLKQRPTPSRDAVVPPYMLDLYRRHSGQPGSPAPDHRLERAASRANTVRSFHHEESLEELPETSGKTTRRFFFNLSSIPTEEFITSAELQVFREQMQDALGNNSSFHHRINIYEIIKPATANSKFPVTSLLDTRLVNQNASRWESFDVTPAVMRWTAQGHANHGFVVEVAHLEEKQGVSKRHVRISRSLHQDEHSWSQIRPLLVTFGHDGKGHPLHKREKRQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR Predict reactive species |
| Full Name | bone morphogenetic protein 2 |
| Calculated Molecular Weight | 396 aa, 44 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC069214 |
| Gene Symbol | BMP2 |
| Gene ID (NCBI) | 650 |
| RRID | AB_2881759 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P12643 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. Two band isofroms of BMP2 are present (PMID: 35846837).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BMP2 antibody 66383-1-Ig | Download protocol |
| WB protocol for BMP2 antibody 66383-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BMP2 Monoclonal antibody (1C5C2), catalog number 66383-1-Ig, has 118 citations. These appear in journals including Nature Communications, Cancer Cell, Advanced Science, Theranostics, ACS Applied Materials & Interfaces, Stem Cell Research & Therapy, and Biomaterials Advances, among others. Associated research fields span bone regeneration and osteogenesis, vascular calcification, osteoporosis, cancer biology, stem cell differentiation, fracture healing, and immunomodulation. Cited applications include Western blot, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Cancer Cell Tumor cell-intrinsic epigenetic dysregulation shapes cancer-associated fibroblasts heterogeneity to metabolically support pancreatic cancer | ||
Ann Rheum Dis Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration | ||
Bioact Mater Continuously released Zn2+ in 3D-printed PLGA/β-TCP/Zn scaffolds for bone defect repair by improving osteoinductive and anti-inflammatory properties | ||
Nat Commun Injectable hydrogels for osteomyelitis treatment induce metabolic reprogramming for protection against reinfection. | ||
J Adv Res Genomic insights into the population history of fat-tailed sheep and identification of two mutations that contribute to fat tail adipogenesis | ||
Redox Biol Small molecule HCY-NBD stabilizes GSTM2 via cys174 sulfenylation to attenuate high glucose induced endothelial cell senescence and calcification. |
