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BMP7 Polyclonal antibody

BMP7 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12221-1-AP

Synonyms

BMP 7, BMP7, bone morphogenetic protein 7, Eptotermin alfa, OP 1, OP1, Osteogenic protein 1



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human kidney tissue, human renal cell carcinoma tissue, human bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12221-1-AP targets BMP7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMP7 fusion protein Ag2902
Full Name bone morphogenetic protein 7
Calculated molecular weight 431 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC008584
Gene symbol BMP7
Gene ID (NCBI) 655
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).

Protocols

Product Specific Protocols
WB protocol for BMP7 antibody 12221-1-APDownload protocol
IHC protocol for BMP7 antibody 12221-1-APDownload protocol
IF protocol for BMP7 antibody 12221-1-APDownload protocol
IP protocol for BMP7 antibody 12221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

EBioMedicine

miR-4739 mediates pleural fibrosis by targeting bone morphogenetic protein 7.

Authors - Meng Wang
humanIHC

Tumour Biol

Prognostic significance of BMP7 as an oncogene in hepatocellular carcinoma.

Authors - Li Wei W
humanWB

Int J Mol Sci

MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e.

Authors - Zhicheng Liu
ratWB

Life Sci

BMP-7 inhibits renal fibrosis in diabetic nephropathy via miR-21 downregulation.

Authors - Lingling Liu
mouseWB

Front Mol Neurosci

Tcf4 Controls Neuronal Migration of the Cerebral Cortex through Regulation of Bmp7.

Authors - Tianda Chen
mouseWB

Circ Res

Yin Yang 1 Suppresses Dilated Cardiomyopathy and Cardiac Fibrosis Through Regulation of Bmp7 and Ctgf.

Authors - Chia Yee Tan