BPGM Polyclonal antibody

BPGM Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17173-1-AP

Synonyms

2,3 bisphosphoglycerate mutase, Bisphosphoglycerate mutase, BPG dependent PGAM, BPGM



Tested Applications

Positive WB detected inmouse liver tissue, human plasma tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

17173-1-AP targets BPGM in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BPGM fusion protein Ag10731
Full Name 2,3-bisphosphoglycerate mutase
Calculated molecular weight 259 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC017050
Gene symbol BPGM
Gene ID (NCBI) 669
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BPGM antibody 17173-1-APDownload protocol
IHC protocol for BPGM antibody 17173-1-APDownload protocol
IP protocol for BPGM antibody 17173-1-APDownload protocol
Standard Protocols
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