BPGM Polyclonal antibody

BPGM Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17173-1-AP

Synonyms

2,3 bisphosphoglycerate mutase, Bisphosphoglycerate mutase, BPG dependent PGAM, BPGM



Tested Applications

Positive WB detected inHEK-293T cells, human plasma, mouse liver tissue, rat liver tissue, HeLa cells, Jurkat cells, K-562 cells, LNCaP cells, U-87 MG cells, mouse heart tissue, mouse lung tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17173-1-AP targets BPGM in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BPGM fusion protein Ag10731
Full Name 2,3-bisphosphoglycerate mutase
Calculated Molecular Weight 259 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC017050
Gene Symbol BPGM
Gene ID (NCBI) 669
RRIDAB_2274991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BPGM antibody 17173-1-APDownload protocol
IHC protocol for BPGM antibody 17173-1-APDownload protocol
IP protocol for BPGM antibody 17173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IP

Cell Metab

Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis.

Authors - Ping Xu
ratWB,IF

Brain Res Bull

Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2

Authors - Guoji E