BRAP Antibody

BRAP Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27431-1-AP

Synonyms

BRAP, BRAP2, BRCA1 associated protein, IMP, RING finger protein 52, RNF52



Tested Applications

Positive WB detected inMCF-7 cells, A431 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

27431-1-AP targets BRAP in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRAP fusion protein Ag26728
Full Name BRCA1 associated protein
Calculated molecular weight 592 aa, 67 kDa
Observed molecular weight 62-70 kDa, 48 kDa
GenBank accession numberBC136698
Gene symbol BRAP
Gene ID (NCBI) 8315
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BRAP antibody 27431-1-APDownload protocol
IHC protocol for BRAP antibody 27431-1-APDownload protocol
IP protocol for BRAP antibody 27431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols