BTG4 Polyclonal antibody

BTG4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20514-1-AP

Synonyms

B cell translocation gene 4, BTG family member 4, BTG4, PC3B, Protein BTG4, Protein PC3b



Tested Applications

Positive WB detected inCaco-2 cells, HeLa cells, mouse colon tissue
Positive IHC detected inhuman ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20514-1-AP is BTG4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTG4 fusion protein Ag14339
Full Name B-cell translocation gene 4
Calculated Molecular Weight 223 aa, 26 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC031045
Gene Symbol BTG4
Gene ID (NCBI) 54766
RRIDAB_10694822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BTG4 antibody 20514-1-APDownload protocol
IHC protocol for BTG4 antibody 20514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols