BTK Polyclonal antibody

BTK Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 21581-1-AP

Synonyms

AGMX1, AT, ATK, B cell progenitor kinase, BPK, Bruton tyrosine kinase, BTK, IMD1, PSCTK1, Tyrosine protein kinase BTK, XLA



Tested Applications

Positive WB detected inDaudi cells, mouse spleen tissue, L02 cells, Ramos cells
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21581-1-AP is BTK Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTK fusion protein Ag16133
Full Name Bruton agammaglobulinemia tyrosine kinase
Calculated molecular weight 659 aa, 76 kDa
Observed molecular weight 70-76 kDa
GenBank accession numberBC109079
Gene symbol BTK
Gene ID (NCBI) 695
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BTK antibody 21581-1-APDownload protocol
IP protocol for BTK antibody 21581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Med

Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus.

Authors - Linlin Sheng