BTK Polyclonal antibody

BTK Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 21581-1-AP

Synonyms

AGMX1, AT, ATK, B cell progenitor kinase, BPK, Bruton tyrosine kinase, BTK, IMD1, PSCTK1, Tyrosine protein kinase BTK, XLA



Tested Applications

Positive WB detected inDaudi cells, Ramos cells, HT-29 cells, NIH/3T3 cells
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

21581-1-AP targets BTK in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTK fusion protein Ag16133
Full Name Bruton agammaglobulinemia tyrosine kinase
Calculated Molecular Weight 659 aa, 76 kDa
Observed Molecular Weight 70-76 kDa
GenBank Accession NumberBC109079
Gene Symbol BTK
Gene ID (NCBI) 695
RRIDAB_10734434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BTK antibody 21581-1-APDownload protocol
IP protocol for BTK antibody 21581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Anoctamin 1 controls bone resorption by coupling Cl- channel activation with RANKL-RANK signaling transduction.

Authors - Weijia Sun
humanWB

Int J Mol Med

Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus.

Authors - Linlin Sheng
mouseWB

Redox Biol

CXCR4-BTK axis mediate pyroptosis and lipid peroxidation in early brain injury after subarachnoid hemorrhage via NLRP3 inflammasome and NF-κB pathway

Authors - Chengli Liu