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ATG14/Barkor (C-terminal) Polyclonal antibody

ATG14/Barkor (C-terminal) Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24412-1-AP

Synonyms

ATG14, Atg14L, Barkor, KIAA0831



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24412-1-AP is ATG14/Barkor (C-terminal) Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG14/Barkor (C-terminal) fusion protein Ag19707
Full Name KIAA0831
Calculated molecular weight 492 aa, 55 kDa
Observed molecular weight 65-68 kDa, 42 kDa
GenBank accession numberBC109090
Gene symbol KIAA0831
Gene ID (NCBI) 22863
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Barkor, also named as ATG14, Atg14L and KIAA0831, is named as Beclin 1-associated autophagy-related key regulator protein. The function of Barkor in autophagy has been manifested in several assays, including stress-induced LC3 lipidation, autophagosome formation, and Salmonella typhimurium amplification. A major band around 65-68 kDa and a light band around 42 kDa can be observed in western blot which may be caused by alternative splicing of ATG14. The specificity of ATG14 antibody has been tested by siRNA test.

Protocols

Product Specific Protocols
WB protocol for ATG14/Barkor (C-terminal) antibody 24412-1-APDownload protocol
IHC protocol for ATG14/Barkor (C-terminal) antibody 24412-1-APDownload protocol
IF protocol for ATG14/Barkor (C-terminal) antibody 24412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Microb Pathog

Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells.

Authors - Qiang Fu