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ATG14/Barkor (N-terminal) Polyclonal antibody

ATG14/Barkor (N-terminal) Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19491-1-AP

Synonyms

ATG14, Atg14L, Barkor, KIAA0831



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19491-1-AP targets ATG14/Barkor (N-terminal) in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name KIAA0831
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 65-68 kDa
GenBank Accession NumberNM_014924
Gene Symbol KIAA0831
Gene ID (NCBI) 22863
RRIDAB_10642701
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).

Protocols

Product Specific Protocols
WB protocol for ATG14/Barkor (N-terminal) antibody 19491-1-APDownload protocol
IHC protocol for ATG14/Barkor (N-terminal) antibody 19491-1-APDownload protocol
IF protocol for ATG14/Barkor (N-terminal) antibody 19491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
humanWB

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou
humanWB

Adv Sci (Weinh)

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Authors - Tiemei Li
humanWB

Autophagy

STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.

Authors - Cefan Zhou
mouseWB

Dev Cell

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Authors - Yuqing Lei
humanWB

Autophagy

Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.

Authors - Xu Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Charlotte (Verified Customer) (01-25-2018)

I do not think that this antibody works properly in U2OS cells. According to the litterature ATG14 is supposed to have a diffuse or punctae pattern and not this huge accumulation near the nucleus.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: U2OS
ATG14/Barkor (N-terminal) Antibody Immunofluorescence, validation (1:100 dilution) in U2OS (Cat no:19491-1-AP)