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ATG14/Barkor (N-terminal) Polyclonal antibody
ATG14/Barkor (N-terminal) Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 19491-1-AP
|Positive WB detected in||HEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
19491-1-AP targets ATG14/Barkor (N-terminal) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||bovine, canine, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||65-68 kDa|
|GenBank accession number||NM_014924|
|Gene ID (NCBI)||22863|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).
|Product Specific Protocols|
|WB protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP||Download protocol|
|IHC protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP||Download protocol|
|IF protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy.
Sequential formation of different layers of dystrophic neurites in Alzheimer's brains.
Bovine viral diarrhea virus infection induces autophagy in MDBK cells.
Onco Targets Ther
MicroRNA-375 Targets ATG14 to Inhibit Autophagy and Sensitize Hepatocellular Carcinoma Cells to Sorafenib.
J Gen Virol
Lentivirus-mediated bta-miR-29b overexpression interferes with bovine viral diarrhea virus replication and viral infection-related autophagy by directly targeting ATG14 and ATG9A in MDBK cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Charlotte (Verified Customer) (01-25-2018)
I do not think that this antibody works properly in U2OS cells. According to the litterature ATG14 is supposed to have a diffuse or punctae pattern and not this huge accumulation near the nucleus.