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  • KD/KO Validated

ATG14/Barkor (N-terminal) Polyclonal antibody

ATG14/Barkor (N-terminal) Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 19491-1-AP

Synonyms

ATG14, Atg14L, Barkor, KIAA0831



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19491-1-AP targets ATG14/Barkor (N-terminal) in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity bovine, dog, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name KIAA0831
Calculated molecular weight 55 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberNM_014924
Gene symbol KIAA0831
Gene ID (NCBI) 22863
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).

Protocols

Product Specific Protocols
WB protocol for ATG14/Barkor (N-terminal) antibody 19491-1-APDownload protocol
IF protocol for ATG14/Barkor (N-terminal) antibody 19491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Mol Cell

Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy.

Authors - Jose Norberto S Vargas
mouseIF

Mol Psychiatry

Sequential formation of different layers of dystrophic neurites in Alzheimer's brains.

Authors - Md Golam Sharoar
bovineWB

J Microbiol

Bovine viral diarrhea virus infection induces autophagy in MDBK cells.

Authors - Qiang Fu
  • KD Validated
humanWB

Cell Cycle

PMID: 31885310

Authors - Zhenhua Hu
humanWB

Onco Targets Ther

MicroRNA-375 Targets ATG14 to Inhibit Autophagy and Sensitize Hepatocellular Carcinoma Cells to Sorafenib.

Authors - Shuo Yang
dogWB

J Gen Virol

Lentivirus-mediated bta-miR-29b overexpression interferes with bovine viral diarrhea virus replication and viral infection-related autophagy by directly targeting ATG14 and ATG9A in MDBK cells.

Authors - Qiang Fu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Charlotte (Verified Customer) (01-25-2018)

I do not think that this antibody works properly in U2OS cells. According to the litterature ATG14 is supposed to have a diffuse or punctae pattern and not this huge accumulation near the nucleus.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: U2OS
ATG14/Barkor (N-terminal) Antibody Immunofluorescence, validation (1:100 dilution) in U2OS (Cat no:19491-1-AP)