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Bcl-XL Polyclonal antibody
Bcl-XL Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 10783-1-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, Raji cells, mouse placenta tissue, HepG2 cells, C6 cells, Jurkat cells, K-562 cells, human placenta tissue, NIH/3T3 cells, RAW264.7, HeLa cells, rat brain tissue|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human lung cancer tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10783-1-AP targets Bcl-XL in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||goat, human, mouse, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Bcl-XL fusion protein Ag1232|
|Full Name||BCL2-like 1|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC019307|
|Gene ID (NCBI)||598|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta) , and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein. This antibody can recognize Bcl-XL, Bcl-X(s) and Bcl-X(Beta).
|Product Specific Protocols|
|WB protocol for Bcl-XL antibody 10783-1-AP||Download protocol|
|IHC protocol for Bcl-XL antibody 10783-1-AP||Download protocol|
|IF protocol for Bcl-XL antibody 10783-1-AP||Download protocol|
|IP protocol for Bcl-XL antibody 10783-1-AP||Download protocol|
|FC protocol for Bcl-XL antibody 10783-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Bastien (Verified Customer) (08-19-2020)
Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with PiP5Ka antibody (15713-1-AP) during 50min After, a seconde staining was performed with 0,05µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody alone and in red primary (10783-1-AP) +secondary antibody