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Bcl-XL Antibody

Bcl-XL Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 10783-1-AP

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL



Tested Applications

Positive WB detected inA549 cells, Raji cells, mouse placenta tissue, HepG2 cells, C6 cells, Jurkat cells, K-562 cells, human placenta tissue, NIH/3T3 cells, RAW264.7, HeLa cells, rat brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10783-1-AP targets Bcl-XL in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag1232
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight 30 kDa
GenBank accession number BC019307
Gene symbol BCL2L1
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta) , and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein. This antibody can recognize Bcl-XL, Bcl-X(s) and Bcl-X(Beta).

Protocols

Product Specific Protocols
WB protocol for Bcl-XL antibody 10783-1-APDownload protocol
IHC protocol for Bcl-XL antibody 10783-1-APDownload protocol
IF protocol for Bcl-XL antibody 10783-1-APDownload protocol
IP protocol for Bcl-XL antibody 10783-1-APDownload protocol
FC protocol for Bcl-XL antibody 10783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Glutaminolysis Mediated by MALT1 Protease Activity Facilitates PD-L1 Expression on ABC-DLBCL Cells and Contributes to Their Immune Evasion.

Authors - Xichun Xia
humanWB

Biochem Biophys Res Commun

Long non-coding RNA FAM84B-AS promotes resistance of gastric cancer to platinum drugs through inhibition of FAM84B expression.

Authors - Yeqian Zhang
ratWB

Transl Neurosci

Protective Effect of Extract of Bletilla Striata on Isoflurane Induced Neuronal Injury By Altering PI3K/Akt Pathway.

Authors - Guoqing Zhao
humanWB

Cell Biol Int

Soluble CXCL16 Promotes TNF-α-induced Apoptosis in DLBCL via the AMAD10-NF-κB Regulatory Feedback Loop.

Authors - Hua Yang
humanWB

Cancer Lett

A novel hydrogen sulfide-releasing donor, HA-ADT, suppresses the growth of human breast cancer cells through inhibiting the PI3K/AKT/mTOR and Ras/Raf/MEK/ERK signaling pathways.

Authors - Qian Dong
humanWB

Biochem Biophys Res Commun

Alisol B-23-acetate, a tetracyclic triterpenoid isolated from Alisma orientale, induces apoptosis in human lung cancer cells via the mitochondrial pathway.

Authors - Jinxia Wang