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  • Featured Product
  • KD/KO Validated

C11orf30 Polyclonal antibody

C11orf30 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26212-1-AP

Synonyms

C11orf30, EMSY, GL002, Protein EMSY

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using C11orf30 Polyclonal antibody (26212-1-AP)

    WB analysis of MCF-7 using 26212-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26212-1-AP (C11orf30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using C11orf30 Polyclonal antibody (26212-1-AP)

    WB analysis of Jurkat using 26212-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26212-1-AP (C11orf30 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using C11orf30 Polyclonal antibody (26212-1-AP)

    IF Staining of MCF-7 using 26212-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26212-1-AP (C11orf30 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, Jurkat cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26212-1-AP is C11orf30 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C11orf30 fusion protein Ag23974
Full Name chromosome 11 open reading frame 30
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC029375
Gene Symbol C11orf30
Gene ID (NCBI) 56946
RRIDAB_2880429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C11orf30 antibody 26212-1-APDownload protocol
IF protocol for C11orf30 antibody 26212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell

EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.

Authors - Antonio Marzio
  • KD Validated
View Article