Tested Applications
| Positive WB detected in | HEK-293T cells, Jurkat cells, HeLa cells, MCF-7 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26212-1-AP targets C11orf30 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23974 Product name: Recombinant human C11orf30 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-171 aa of BC029375 Sequence: KKALATSMLTGEAGSLPSTHMVVAGMANSTPQQQKCRESCSSPSTVGSSLTTRKIDPPAVPATGQFMRIQNVGQKKAEESPAEIIIQAIPQYAIPCHSSSNVVVEPSGLLELNNFTSQQLDDEETAMEQDIDSSTEDGTEPSPSQSSAERS Predict reactive species |
| Full Name | chromosome 11 open reading frame 30 |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC029375 |
| Gene Symbol | C11orf30 |
| Gene ID (NCBI) | 56946 |
| RRID | AB_2880429 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z589 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for C11orf30 antibody 26212-1-AP | Download protocol |
| WB protocol for C11orf30 antibody 26212-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EMSY antibody (Cat# 26212-1-AP) has 2 total citations. These appear in Cell and European Journal of Medical Research. The research fields cover lung cancer immune evasion—specifically EMSY's role in inhibiting homologous recombination repair and the interferon response—and ovarian cancer metabolism, examining how EMSY enhances glycolysis in ovarian cancer cells. Both studies utilized WB and IHC applications in human samples.
| Species | Application | Title |
|---|---|---|
Cell EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.
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