• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

C1orf27 Polyclonal antibody

C1orf27 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22844-1-AP

Synonyms

C1orf27, hODR 4, LAG1 interacting protein, odr 4, ODR4, Protein odr 4 homolog, TTG1, TTG1A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using C1orf27 Polyclonal antibody (22844-1-AP)

    WB analysis of mouse brain using 22844-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22844-1-AP (C1orf27 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using C1orf27 Polyclonal antibody (22844-1-AP)

    WB analysis of NIH/3T3 using 22844-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22844-1-AP (C1orf27 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C1orf27 Polyclonal antibody (22844-1-AP)

    IHC staining of human breast cancer using 22844-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22844-1-AP (C1orf27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C1orf27 Polyclonal antibody (22844-1-AP)

    IHC staining of human breast cancer using 22844-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22844-1-AP (C1orf27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22844-1-AP is C1orf27 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1orf27 fusion protein Ag18802
Full Name chromosome 1 open reading frame 27
Calculated Molecular Weight 454 aa, 51 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC003397
Gene Symbol C1orf27
Gene ID (NCBI) 54953
RRIDAB_2879176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C1orf27 antibody 22844-1-APDownload protocol
IHC protocol for C1orf27 antibody 22844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

J Biol Chem

Human C1orf27 protein interacts with α2A-adrenergic receptor and regulates its anterograde transport.

Authors - Xin Xu
  • KD Validated
View Article