C2orf79/PTRHD1 Polyclonal antibody

C2orf79/PTRHD1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25576-1-AP

Synonyms

C2orf79, PTRHD1



Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25576-1-AP is C2orf79/PTRHD1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C2orf79/PTRHD1 fusion protein Ag22255
Full Name chromosome 2 open reading frame 79
Calculated molecular weight 140 aa, 16 kDa
Observed molecular weight 13-16 kDa
GenBank accession numberBC073803
Gene symbol C2orf79
Gene ID (NCBI) 391356
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IHC protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IF protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
IP protocol for C2orf79/PTRHD1 antibody 25576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols