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C3orf64 Polyclonal antibody

C3orf64 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27595-1-AP

Synonyms

AER61, C3orf64, EGF domain-specific O-linked GlcNAc transferase, EOGT, EOGT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C3orf64 Polyclonal antibody (27595-1-AP)

    WB analysis using 27595-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27595-1-AP (C3orf64 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using C3orf64 Polyclonal antibody (27595-1-AP)

    IP experiment of mouse brain using 27595-1-AP

    IP result of anti-C3orf64 (IP:27595-1-AP, 4ug; Detection:27595-1-AP 1:500) with mouse brain tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C3orf64 Polyclonal antibody (27595-1-AP)

    IHC staining of human breast cancer using 27595-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27595-1-AP (C3orf64 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C3orf64 Polyclonal antibody (27595-1-AP)

    IHC staining of human breast cancer using 27595-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27595-1-AP (C3orf64 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C3orf64 Polyclonal antibody (27595-1-AP)

    IHC staining of human liver cancer using 27595-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27595-1-AP (C3orf64 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C3orf64 Polyclonal antibody (27595-1-AP)

    IHC staining of human liver cancer using 27595-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27595-1-AP (C3orf64 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, NIH/3T3 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27595-1-AP targets C3orf64 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C3orf64 fusion protein Ag25932
Full Name chromosome 3 open reading frame 64
Observed Molecular Weight 60-62 kDa
GenBank Accession NumberBC060887
Gene Symbol C3orf64
Gene ID (NCBI) 285203
RRIDAB_2880919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C3orf64 antibody 27595-1-APDownload protocol
IHC protocol for C3orf64 antibody 27595-1-APDownload protocol
IP protocol for C3orf64 antibody 27595-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
View Article
humanWB

Adv Sci (Weinh)

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Authors - Tiemei Li
View Article
humanWB,IF,IP

Genomics

SHCBP1 interacting with EOGT enhances O-GlcNAcylation of NOTCH1 and promotes the development of pancreatic cancer.

Authors - Can Yang
  • KD Validated
View Article
humanWB

Proteomics

Solvent-induced proteome profiling for proteomic quantitation and target discovery of small molecular drugs

Authors - Chengli Yu
View Article