Filter: AllWBIHCIF/ICC

WB analysis of MCF-7 using 25124-1-AP
WB result of C6orf211/ARMT1 antibody (25124-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C6orf211/ARMT1 transfected MCF-7 cells.
WB analysis of MCF-7 using 25124-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 25124-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of T-47D using 25124-1-AP
T-47D cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 25124-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 25124-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25124-1-AP (C6orf211 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 25124-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25124-1-AP (C6orf211 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 25124-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 25124-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 25124-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 25124-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of T-47D using 25124-1-AP
Immunofluorescent analysis of (4% PFA) fixed T-47D cells using C6orf211/ARMT1 antibody (25124-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Featured Product
KD/KO Validated

C6orf211/ARMT1 Polyclonal antibody

Name: C6orf211/ARMT1 Polyclonal antibody
Brand: Proteintech
SKU: 25124-1-AP

C6orf211/ARMT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25124-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C6orf211, ARMT1, Sugar phosphate phosphatase ARMT1, EC:3.1.3.-, EC:2.1.1.-

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	MCF-7 cells, HEK-293 cells, A431 cells, T-47D cells
Positive IHC detected in	human breast cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	T-47D cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25124-1-AP targets C6orf211/ARMT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19090 Product name: Recombinant human C6orf211 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 91-439 aa of BC011348 Sequence: YLEYQQSLLNESDGKSRWFYSPWLLVECYIDRRIHEAIIQSPPIDYFDVFKESKEQNFYGSQESIIALCTHLQQLIRTIEDLYENQLKDEFFKLLQISLWGNKCDLSLSGGESSSQNTNVLNSLEDLKPFILLNDMEHLWSLLSNCKKTREKASATRVYIVLDNSGFELVTDLILADFLLSSELATEVHFYGKTIPWFVSDTTIHDFNWLIEQVKHSNHKWMSKCGADWEEYIKMGKWVYHNHIFWTLPHEYCAMPQVAPDLYAELQKAHLILFKGDLNYRKLTGDRKWEFSVPFHQALNGFHPAPLCTIRTLKAEIQVGLQPGQGEQLLASEPSWWTTGKYGIFQYDG Predict reactive species
Full Name	chromosome 6 open reading frame 211
Calculated Molecular Weight	441 aa, 51 kDa
Observed Molecular Weight	50-51 kDa
GenBank Accession Number	BC011348
Gene Symbol	C6orf211/ARMT1
Gene ID (NCBI)	79624
RRID	AB_2879911
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H993
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for C6orf211/ARMT1 antibody 25124-1-AP	Download protocol
IHC protocol for C6orf211/ARMT1 antibody 25124-1-AP	Download protocol
WB protocol for C6orf211/ARMT1 antibody 25124-1-AP	Download protocol