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CACNG3 Polyclonal antibody

CACNG3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13729-1-AP

Synonyms

Cacng2, CACNG3

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CACNG3 Polyclonal antibody (13729-1-AP)

    WB analysis of HEK-293 using 13729-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13729-1-AP (CACNG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using CACNG3 Polyclonal antibody (13729-1-AP)

    WB analysis of mouse kidney using 13729-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13729-1-AP (CACNG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CACNG3 Polyclonal antibody (13729-1-AP)

    IHC staining of mouse brain using 13729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13729-1-AP (CACNG3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CACNG3 Polyclonal antibody (13729-1-AP)

    IHC staining of mouse brain using 13729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13729-1-AP (CACNG3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13729-1-AP targets CACNG3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CACNG3 fusion protein Ag4730
Full Name calcium channel, voltage-dependent, gamma subunit 3
Calculated Molecular Weight 315 aa, 36 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC040005
Gene Symbol CACNG3
Gene ID (NCBI) 10368
RRIDAB_2070085
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Voltage-dependent calcium channels couple membrane depolarization in a number of cellular processes. These activities are regulated by distinct channels composed of alpha-1, beta, alpha-2/delta, and gamma subunits. CACNG3, one of the eight gamma subunits in human, is a type I transmembrane AMPA receptor regulatory protein (TARP). TARPs regulate both trafficking and channel gating of the AMPA receptors.

Protocols

Product Specific Protocols
WB protocol for CACNG3 antibody 13729-1-APDownload protocol
IHC protocol for CACNG3 antibody 13729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

BMC Cancer

Integrated profiling identifies CACNG3 as a prognostic biomarker for patients with glioma

Authors - Enfang Shan
View Article