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- KD/KO Validated
CALCOCO2 Polyclonal antibody
CALCOCO2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12229-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, rat skeletal muscle tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 11 publications below |
| IF | See 2 publications below |
Product Information
12229-1-AP targets CALCOCO2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, swine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CALCOCO2 fusion protein Ag2866 |
| Full Name | calcium binding and coiled-coil domain 2 |
| Calculated molecular weight | 446 aa, 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC015893 |
| Gene symbol | CALCOCO2 |
| Gene ID (NCBI) | 10241 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CALCOCO2, also named as NDP52, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat Calcoco2 has some isoforms with MW 28-40 kDa and 67 kDa. Human CALCOCO2 has some isoforms with MW 43-47 kDa and 52-55 kDa,
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CALCOCO2 antibody 12229-1-AP | Download protocol |
| IHC protocol for CALCOCO2 antibody 12229-1-AP | Download protocol |
| IF protocol for CALCOCO2 antibody 12229-1-AP | Download protocol |
| IP protocol for CALCOCO2 antibody 12229-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Beclin1-driven autophagy modulates the inflammatory response of microglia via NLRP3. | ||
Nat Commun A selective inhibitor of mitofusin 1-βIIPKC association improves heart failure outcome in rats. | ||
Oncogene Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab. | ||
Mol Cell TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress. | ||
Chem Biol Interact Activation of PINK1-Parkin-dependent mitophagy in Tri-ortho-cresyl phosphate-treated Neuro2a cells. | ||
Commun Biol Streptococcus pneumoniae triggers hierarchical autophagy through reprogramming of LAPosome-like vesicles via NDP52-delocalization. |
