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CALCOCO2 Polyclonal antibody

CALCOCO2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12229-1-AP

Synonyms

CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52



Tested Applications

Positive WB detected inHeLa cells, rat skeletal muscle tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12229-1-AP targets CALCOCO2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALCOCO2 fusion protein Ag2866
Full Name calcium binding and coiled-coil domain 2
Calculated molecular weight 446 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC015893
Gene symbol CALCOCO2
Gene ID (NCBI) 10241
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CALCOCO2, also named as NDP52, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat Calcoco2 has some isoforms with MW 28-40 kDa and 67 kDa. Human CALCOCO2 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Protocols

Product Specific Protocols
WB protocol for CALCOCO2 antibody 12229-1-APDownload protocol
IHC protocol for CALCOCO2 antibody 12229-1-APDownload protocol
IF protocol for CALCOCO2 antibody 12229-1-APDownload protocol
IP protocol for CALCOCO2 antibody 12229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

EMBO J

Beclin1-driven autophagy modulates the inflammatory response of microglia via NLRP3.

Authors - Judith Houtman
WB

Nat Commun

A selective inhibitor of mitofusin 1-βIIPKC association improves heart failure outcome in rats.

Authors - Julio C B Ferreira
humanWB

Oncogene

Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab.

Authors - Dawei Wang

Mol Cell

TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.

Authors - Heeseon An
mouseWB

Chem Biol Interact

Activation of PINK1-Parkin-dependent mitophagy in Tri-ortho-cresyl phosphate-treated Neuro2a cells.

Authors - Yu Wang
humanWB

Commun Biol

Streptococcus pneumoniae triggers hierarchical autophagy through reprogramming of LAPosome-like vesicles via NDP52-delocalization.

Authors - Michinaga Ogawa