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  • KD/KO Validated

CaMKII alpha-Specific Antibody

CaMKII alpha-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20666-1-AP

Synonyms

CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMKA, CaMKII alpha, KIAA0968



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

20666-1-AP targets CaMKII alpha-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name calcium/calmodulin-dependent protein kinase II alpha
Calculated molecular weight 55 kDa
Observed molecular weight 50 kDa
GenBank accession number NM_015981
Gene symbol CAMK2A
Gene ID (NCBI) 815
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CaMKII alpha, also named as CAMKA and KIAA0968, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody is specific to CAMK2A.

Protocols

Product Specific Protocols
WB protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
IHC protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
IF protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
IP protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biomed Res Int

Proteomic Profile of Carbonylated Proteins Screen Regulation of Apoptosis via CaMK Signaling in Response to Regular Aerobic Exercise.

Authors - Wenfeng Liu
mouseWB

Dis Model Mech

Loss of Frrs1l disrupts synaptic AMPA receptor function, and results in neurodevelopmental, motor, cognitive and electrographical abnormalities.

Authors - Michelle Stewart
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
  • KD Validated
mouseWB

ACS Chem Neurosci

Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.

Authors - Lei Wu
ratWB

J Neuropathol Exp Neurol

Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.

Authors - Wenfeng Liu
humanWB

Int J Biol Sci

Wnt5a/CaMKII/ERK/CCL2 axis is required for tumor-associated macrophages to promote colorectal cancer progression.

Authors - Qing Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jonathan (Verified Customer) (11-11-2019)

Works well for WB (1:1000)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
No image submitted with review