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CAP2 Monoclonal antibody

CAP2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G7D2

Cat no : 67412-1-Ig

Synonyms

CAP 2, CAP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis using 67412-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HepG2 cells using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis of HepG2 using 67412-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis of PC-3 using 67412-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis of COLO 320 using 67412-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis of A549 using 67412-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CAP2 Monoclonal antibody (67412-1-Ig)

    WB analysis of HeLa using 67412-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, PC-3 cells, COLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67412-1-Ig targets CAP2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CAP2 fusion protein Ag8622
Full Name CAP, adenylate cyclase-associated protein, 2 (yeast)
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 51-53 kDa
GenBank Accession NumberBC008481
Gene Symbol CAP2
Gene ID (NCBI) 10486
RRIDAB_2882653
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPs (CAP1 and CAP2) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is predominantly expressed in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Overexpression of CAP2 has been reported in many cancers, including hepatocellular carcinoma (HCC), human breast cancer, and malignant melanoma.

Protocols

Product Specific Protocols
WB protocol for CAP2 antibody 67412-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols