|Positive WB detected in||Jurkat cells, HeLa cells, HL-60 cells, Staurosporine treated Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human heart tissue, mouse lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:300-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10380-1-AP targets Caspase 9/p35/p10 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Astragalus membranaceus, bovine, goat, hamster, human, mouse, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Caspase 9/p35/p10 fusion protein Ag0404|
|Full Name||caspase 9, apoptosis-related cysteine peptidase|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||46 kDa, 35 kDa|
|GenBank accession number||BC002452|
|Gene ID (NCBI)||842|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9.
|Product Specific Protocols|
|WB protocol for Caspase 9/p35/p10 antibody 10380-1-AP||Download protocol|
|IHC protocol for Caspase 9/p35/p10 antibody 10380-1-AP||Download protocol|
|IF protocol for Caspase 9/p35/p10 antibody 10380-1-AP||Download protocol|
|IP protocol for Caspase 9/p35/p10 antibody 10380-1-AP||Download protocol|
|FC protocol for Caspase 9/p35/p10 antibody 10380-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
WEI (Verified Customer) (01-17-2020)
Good to detect apoptosis in mouse heart subject to IR injury.