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c-Cbl Polyclonal antibody

c-Cbl Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25818-1-AP

Synonyms

C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55



Tested Applications

Positive WB detected inK-562 cells, Raji cells, Jurkat cells, Ramos cells, Romas cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25818-1-AP is c-Cbl Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-Cbl fusion protein Ag23033
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC132733
Gene symbol CBL
Gene ID (NCBI) 867
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for c-Cbl antibody 25818-1-APDownload protocol
IHC protocol for c-Cbl antibody 25818-1-APDownload protocol
IP protocol for c-Cbl antibody 25818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oxid Med Cell Longev

Salidroside-Mediated Autophagic Targeting of Active Src and Caveolin-1 Suppresses Low-Density Lipoprotein Transcytosis across Endothelial Cells.

Authors - Xiangli Bai
humanWB,IF

Hepatology

PMID: 29729186

Authors - Huixia Zhang
  • KD Validated