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CCAR2 Monoclonal antibody

CCAR2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D10F8

Cat no : 66497-1-Ig

Synonyms

CCAR2, cell cycle and apoptosis regulator 2, DBC 1, DBC.1, DBC1, KIAA1967, p30 DBC, Protein KIAA1967



Tested Applications

Positive WB detected inHeLa cells, pig brain tissue, HEK-293 cells
Positive IHC detected inhuman cervical cancer tissue, human lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66497-1-Ig targets CCAR2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCAR2 fusion protein Ag18374
Full Name KIAA1967
Calculated molecular weight 923 aa, 103 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC018269
Gene symbol KIAA1967
Gene ID (NCBI) 57805
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).

Protocols

Product Specific Protocols
WB protocol for CCAR2 antibody 66497-1-IgDownload protocol
IHC protocol for CCAR2 antibody 66497-1-IgDownload protocol
IF protocol for CCAR2 antibody 66497-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols