Filter:
Able AI
What's the best dilution for CCR8 in ?
Tested Applications
| Positive WB detected in | A431 cells, A549 cells, HepG2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84545-1-RR targets CCR8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | chemokine (C-C motif) receptor 8 |
| Calculated Molecular Weight | 355 aa, 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC074743 |
| Gene Symbol | CCR8 |
| Gene ID (NCBI) | 1237 |
| RRID | AB_3672050 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P51685 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCR8 antibody 84545-1-RR | Download protocol |
| WB protocol for CCR8 antibody 84545-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CCR8 Recombinant monoclonal antibody (241663E4), catalog number 84545-1-RR, has 1 total citation. The cited publication appeared in PLoS Neglected Tropical Diseases and investigated how CCR8 orchestrates an immunosuppressive niche in the liver to promote Echinococcus multilocularis infection. The research field encompasses immunology and parasitic infectious disease. The antibody was applied in IHC and IF for mouse samples in this study.
| Species | Application | Title |
|---|---|---|
PLoS Negl Trop Dis CCR8 orchestrates an immunosuppressive niche in the liver to promote Echinococcus multilocularis infection. |
