CCRL2 Polyclonal antibody

CCRL2 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 13387-1-AP

Synonyms

C C chemokine receptor like 2, CCR11, CCR6, CCRL2, Chemokine receptor CCR11, Chemokine receptor X, CKRX, CRAM, CRAM A, CRAM B, HCR



Tested Applications

Positive WB detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13387-1-AP targets CCRL2 in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCRL2 fusion protein Ag4045
Full Name chemokine (C-C motif) receptor-like 2
Calculated Molecular Weight 344 aa, 40 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC025717
Gene Symbol CCRL2
Gene ID (NCBI) 9034
RRIDAB_2073388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chemokine CC motif receptor-like 2 (CCRL2) is a seven-transmembrane domain receptor that shares structural and functional similarities with the family of atypical chemokine receptors (ACKRs) (PMID: 28743719). CCRL2 does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. CCRL2 has been found on almost all hemopoietic cells, including monocytes, macrophages, polymorphonuclear neutrophils (PMNs), T cells, dendritic cells, natural killer cells, and CD34+ progenitor cells, with PMNs expressing the highest levels (PMID: 15188357). CCRL2 is also expressed by barrier cells, such as lymphatic and blood endothelial cells and bronchial and intestinal epithelial cells (PMID: 29056935).

Protocols

Product Specific Protocols
WB protocol for CCRL2 antibody 13387-1-APDownload protocol
FC protocol for CCRL2 antibody 13387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Aging (Albany NY)

RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.

Authors - Chan Chen
ratWB

Pflugers Arch

Chemerin-9-induced contraction was enhanced through the upregulation of smooth muscle chemokine-like receptor 1 in isolated pulmonary artery of pulmonary arterial hypertensive rats.

Authors - Ayaho Omori