CCT4 Polyclonal antibody

CCT4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21524-1-AP

Synonyms

CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta



Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21524-1-AP is CCT4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCT4 fusion protein Ag16128
Full Name chaperonin containing TCP1, subunit 4 (delta)
Calculated molecular weight 539 aa, 58 kDa
Observed molecular weight 58-60 kDa
GenBank accession numberBC106934
Gene symbol CCT4
Gene ID (NCBI) 10575
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCT4 antibody 21524-1-APDownload protocol
IHC protocol for CCT4 antibody 21524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Pathog Dis

Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.

Authors - Hirai Kimito K