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  • KD/KO Validated

CD100 Polyclonal antibody

CD100 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14422-1-AP

Synonyms

A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D



Tested Applications

Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14422-1-AP targets CD100 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD100 fusion protein Ag5769
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Calculated molecular weight 96 kDa
GenBank accession numberBC054500
Gene symbol SEMA4D
Gene ID (NCBI) 10507
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor metastasis.

Protocols

Product Specific Protocols
IHC protocol for CD100 antibody 14422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Dent Res

Prevention of Alveolar Bone Loss in an Osteoporotic Animal Model via Interference of Semaphorin 4d.

Authors - Y Zhang
  • KD Validated

Sci Rep

Bone scaffolds loaded with siRNA-Semaphorin4d for the treatment of osteoporosis related bone defects.

Authors - Yufeng Zhang
mouseWB

J Bone Miner Res

Anabolic Bone Formation Via a Site Specific Bone Targeting Delivery System by Interfering with Semaphorin 4D Expression.

Authors - Yufeng Zhang