Tested Applications
| Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue, mouse colon tissue, rat colon tissue, rat thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | mouse spleen tissue |
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27949-1-AP targets CD19 in WB, IHC, IF/ICC, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27656 Product name: Recombinant human CD19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 456-556 aa of BC006338 Sequence: SNAESYENEDEELTQPVARTMDFLSPHGSAWDPSREATSLGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR Predict reactive species |
| Full Name | CD19 molecule |
| Calculated Molecular Weight | 556 aa, 61 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC006338 |
| Gene Symbol | CD19 |
| Gene ID (NCBI) | 930 |
| ENSEMBL Gene ID | ENSG00000177455 |
| RRID | AB_2881019 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15391 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD19 antibody 27949-1-AP | Download protocol |
| IHC protocol for CD19 antibody 27949-1-AP | Download protocol |
| WB protocol for CD19 antibody 27949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD19 Polyclonal antibody (Cat# 27949-1-AP) has 28 citations published across 26 journals, including Cell Mol Immunol, Cancer Lett, Front Immunol, FASEB J, Adv Sci, Biomaterials, and J Natl Cancer Cent. Associated research fields span immunology, oncology, inflammation, B-cell biology, HIV, neurology, bone regeneration, and liver disease.
| Species | Application | Title |
|---|---|---|
J Natl Cancer Cent The single-cell immune landscape of HIV-associated aggressive B-cell lymphoma | ||
Cell Mol Immunol Oncolytic virus M1 reinvigorates CD8(+) T-cell immunity against glioblastoma through B-cell-dependent antigen cross-presentation in the spleen. | ||
Bioact Mater TLR2 inhibition attenuates NET-driven inflammation and restenosis during poly-lactic acid bioresorbable scaffold degradation. | ||
J Adv Res Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model | ||
J Nanobiotechnology Targeting CD39 boosts PD-1 blockade antitumor therapeutic efficacy via strengthening CD8 + TILs function and recruiting B cells in cervical cancer | ||
Adv Sci (Weinh) Sleep Deprivation Aggravates Periodontitis Through Trigeminal-Periodontal Neuroimmune Pathway Mediated by the AChE-ACh-α7nAChR Axis. |
Reviews
What customers say
Based on available reviews, this antibody received a 5-star rating from a user who employed it at a 1:50 dilution for immunofluorescence on vaginal mucosa tissue. The reviewer described it as a great antibody that performed well on what they characterized as a tricky tissue type, indicating reliable staining performance even in challenging samples. Overall, the feedback is positive, highlighting effective results in difficult applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shannon (Verified Customer) (06-04-2026) | Great antibody, staining worked well on a tricky tissue type.
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