Tested Applications
| Positive WB detected in | J774A.1 cells |
| Positive IHC detected in | mouse lung tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86552-3-RR targets CD204 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg4727 Product name: Recombinant Mouse MSR1 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 79-458 aa of NM_001113326.1 Sequence: QLLNWEMKNCLVCSLNTSDTSQGPMEKENTSKVEMRFTIIMEHMKDMEERIESISNSKADLIDTERFQNFSMATDQRLNDILLQLNSLISSVQEHGNSLDAISKSLQSLNMTLLDVQLHTETLNVRVRESTAKQQEDISKLEERVYKVSAEVQSVKEEQAHVEQEVKQEVRVLNNITNDLRLKDWEHSQTLKNITFIQGPPGPQGEKGDRGLTGQTGPPGAPGIRGIPGVKGDRGQIGFPGGRGNPGAPGKPGRSGSPGPKGQKGEKGSVGGSTPLKTVRLVGGSGAHEGRVEIFHQGQWGTICDDRWDIRAGQVVCRSLGYQEVLAVHKRAHFGQGTGPIWLNEVMCFGRESSIENCKINQWGVLSCSHSEDAGVTCTS Predict reactive species |
| Full Name | macrophage scavenger receptor 1 |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 70-90 kDa |
| GenBank Accession Number | NM_001113326.1 |
| Gene Symbol | Msr1 |
| Gene ID (NCBI) | 20288 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P30204-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MSR1, also known as scavenger receptor-A (SR-A) or cluster of differentiation 204 (CD204), was first described in 1979 by Brown and Goldstein. MSR1 is highly expressed in lung, arteries, and adipose tissue.MSR1 is predominantly expressed in various macrophage cell types and is mainly altered in macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, host defence, and cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD204 antibody 86552-3-RR | Download protocol |
| WB protocol for CD204 antibody 86552-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
