CD247 Polyclonal antibody

CD247 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12837-2-AP

Synonyms

CD247, CD247 molecule, CD3 ZETA, CD3H, CD3Q, CD3Z, T cell receptor T3 zeta chain, T3Z, TCRZ



Tested Applications

Positive WB detected inJurkat cells, human spleen tissue, mouse spleen tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12837-2-AP targets CD247 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD247 fusion protein Ag3889
Full Name CD247 molecule
Calculated molecular weight 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC025703
Gene symbol CD247
Gene ID (NCBI) 919
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD247, also known as CD3Z (T-cell surface glycoprotein CD3 zeta chain), belongs to the CD3Z/FCER1G family. CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Protocols

Product Specific Protocols
WB protocol for CD247 antibody 12837-2-APDownload protocol
IHC protocol for CD247 antibody 12837-2-APDownload protocol
IF protocol for CD247 antibody 12837-2-APDownload protocol
IP protocol for CD247 antibody 12837-2-APDownload protocol
FC protocol for CD247 antibody 12837-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

Liver Sinusoidal Endothelial Cell-Mediated CD8 T Cell Priming Depends on Co-Inhibitory Signal Integration over Time.

Authors - Julita Kaczmarek

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Haizhen (Verified Customer) (10-15-2019)

specific for human CD3 zeta protein

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000 for western blot
  • Cell Tissue Type: leukemia cell line