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CoraLite® Plus 488-conjugated CD31 Monoclonal antibody

CD31 Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F8E2

Cat no : CL488-66065

Synonyms

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Lymphatic endothelial cells cells using CoraLite® Plus 488-conjugated CD31 Monoclonal anti (CL488-66065)

    IF Staining of Lymphatic endothelial cells using CL488-66065

    Lymphatic endothelial cells were stained for CD31 (green, Cat. No CL488-66065, 1:200) and alpha tubulin (magenta, Cat. 11224-1-AP, 1:200) with DAPI as a counterstain for visualizing the nucleus (yellow). CD31 is an endothelial cell adhesion protein and was conjugated to CoraLite-488 fluorescent dye to allow for direct visualization. Alpha tubulin is a cytoskeletal protein important in cell division and migration and was visualized with an Alexa Fluor 568 secondary antibody. Cells were fixed with 4% paraformaldehyde. Image credit: @Immunofluorescence on Instagram

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated CD31 Monoclonal anti (CL488-66065)

    IF Staining of human tonsillitis using CL488-66065

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD31 antibody (CL488-66065, Clone: 2A1E2 ) at dilution of 1:400.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated CD31 Monoclonal anti (CL488-66065)

    IF Staining of human colon cancer using CL488-66065

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 CD31 antibody (CL488-66065, Clone: 3F8E2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated CD31 Monoclonal anti (CL488-66065)

    IF Staining of human colon cancer using CL488-66065

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 CD31 antibody (CL488-66065, Clone: 3F8E2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of THP-1 cells using CoraLite® Plus 488-conjugated CD31 Monoclonal anti (CL488-66065)

    FC experiment of THP-1 using CL488-66065

    1X10^6 THP-1 cells were surface stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD31 (CL488-66065, Clone:3F8E2) (red), or 0.4 ug Control Antibody. Cells were not fixed.

Tested Applications

Positive IF detected inLymphatic endothelial cells cells, human colon cancer tissue, human tonsillitis tissue
Positive FC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

CL488-66065 targets CD31 in IF, FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD31 fusion protein Ag1787
Full Name platelet/endothelial cell adhesion molecule
Calculated Molecular Weight 83 kDa
GenBank Accession NumberBC022512
Gene Symbol PECAM1
Gene ID (NCBI) 5175
RRIDAB_2883247
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489). This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD31 antibody CL488-66065Download protocol
FC protocol for CL Plus 488 CD31 antibody CL488-66065Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Biochem Biophys Res Commun

Irisin links Claudin-5 preservation and Mfn2-mediated mitochondrial dynamics to resist doxorubicin-induced cardiac endothelial damage

Authors - Jun He
View Article
humanIF

Cell Death Dis

Exosomal PGAM1 promotes prostate cancer angiogenesis and metastasis by interacting with ACTG1

Authors - Jun-Qi Luo
View Article