CD34 Recombinant monoclonal antibody

CD34 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 84242-5-RR

Clone No.242069F8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

242069F8, CD34 molecule

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Filter: AllWBIHC-AutostainerIHCIF-PAffinity

WB analysis using 84242-5-RR
Various lysates were subjected to SDS PAGE followed by western blot with 84242-5-RR (CD34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colorectal cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colorectal cancer using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 84242-5-RR
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human placenta using 84242-5-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD34 antibody (84242-5-RR, Clone: 242069F8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Affinity and Kinetic Characterization of 84242-5-RR
Biolayer interferometry (BLl) kinetic assays of 84242-5-RR against Human CD34 were performed. The affinity constant is 16.3 nM.

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HL-60 cells, bEnd.3 cells, HUVEC cells, mouse testis tissue, human placenta tissue
Positive IHC detected in	human tonsillitis tissue, human colon cancer tissue, human liver cancer tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human placenta tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84242-5-RR targets CD34 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Recombinant protein Predict reactive species
Full Name	CD34 molecule
Calculated Molecular Weight	41 kDa
Observed Molecular Weight	105 kDa
GenBank Accession Number	BC039146
Gene Symbol	CD34
Gene ID (NCBI)	947
RRID	AB_3743536
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P28906
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Protocols

Product Specific Protocols
IF protocol for CD34 antibody 84242-5-RR	Download protocol
IHC protocol for CD34 antibody 84242-5-RR	Download protocol
WB protocol for CD34 antibody 84242-5-RR	Download protocol