CD37 Antibody

CD37 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21044-1-AP

Synonyms

CD37, CD37 molecule, GP52 40, Leukocyte antigen CD37, Tetraspanin 26, Tspan 26, TSPAN26



Tested Applications

Positive WB detected inrat spleen tissue, HeLa cells, human spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 21044-1-AP immunogen is Fusion Protein CD37 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD37 fusion protein Ag15384
Full Name CD37 molecule
Calculated molecular weight 281aa,32 kDa
Observed molecular weight 40-52 kDa
GenBank accession number BC110379
Gene symbol CD37
Gene ID (NCBI) 951
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).

Protocols

Product Specific Protocols
WB protocol for CD37 antibody 21044-1-APDownload protocol
IHC protocol for CD37 antibody 21044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols