CD3 Gamma Monoclonal antibody

CD3 Gamma Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2E9G7

Cat no : 60347-1-Ig

Synonyms

CD3 gamma, CD3G, T cell receptor T3 gamma chain, T3G



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60347-1-Ig targets CD3 Gamma in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD3 Gamma fusion protein Ag15502
Full Name CD3g molecule, gamma (CD3-TCR complex)
Calculated molecular weight 182 aa, 20 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC113830
Gene symbol CD3G
Gene ID (NCBI) 917
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Protocols

Product Specific Protocols
WB protocol for CD3 Gamma antibody 60347-1-IgDownload protocol
IHC protocol for CD3 Gamma antibody 60347-1-IgDownload protocol
FC protocol for CD3 Gamma antibody 60347-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols