CD4 Polyclonal antibody
CD4 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 19068-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse thymus tissue |
| Positive IP detected in | mouse thymus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
19068-1-AP targets CD4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD4 fusion protein Ag13616 |
| Full Name | CD4 molecule |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC025782 |
| Gene symbol | CD4 |
| Gene ID (NCBI) | 920 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD4 antibody 19068-1-AP | Download protocol |
| IP protocol for CD4 antibody 19068-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Med The preoperative neutrophil-to-lymphocyte ratio is not a marker of prostate cancer characteristics but is an independent predictor of biochemical recurrence in patients receiving radical prostatectomy. | ||
Mol Med Rep Rebamipide ophthalmic solution modulates the ratio of T helper cell 17/regulatory T cells in dry eye disease mice. | ||
Biochem Cell Biol The combination of indirubin and isatin attenuates dextran sodium sulfate-induced ulcerative colitis in mice. | ||
J Hematol Oncol PD-1 axis expression in musculoskeletal tumors and antitumor effect of nivolumab in osteosarcoma model of humanized mouse. |
