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CD4 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 19068-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

CD4 Antibody WB mouse thymus tissue 19068-1-AP

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19068-1-AP(CD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CD4 Antibody IP mouse thymus tissue 19068-1-AP

IP Result of anti-CD4 (IP:19068-1-AP, 4ug; Detection:19068-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

CD4 Antibody IHC human tonsillitis tissue 19068-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 19068-1-AP( CD4 Antibody) at dilution of 1:100 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD4 Antibody IHC human tonsillitis tissue 19068-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 19068-1-AP( CD4 Antibody) at dilution of 1:100 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
mouse thymus tissue
Positive IP detected in:
mouse thymus tissue
Positive IHC detected in:
human tonsillitis tissue
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD4 fusion protein Ag13616
Full name:
CD4 molecule
Calculated molecular weight:
55 kDa
Observed molecular weight:
55 kDa
GenBank accession number:
BC025782
Gene ID (NCBI):
920
Gene symbol
CD4
Synonyms
CD4, CD4 molecule, CD4mut

Background

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).


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