CD40L/CD154 Antibody

CD40L/CD154 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1E6D10

Cat no : 66502-1-Ig

Synonyms

CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

66502-1-Ig targets CD40L/CD154 in WB, IHC, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD40L/CD154 fusion protein Ag24941
Full Name CD40 ligand
Calculated molecular weight 261aa,29 kDa
Observed molecular weight 32 kDa
GenBank accession number BC071754
Gene symbol CD40LG
Gene ID (NCBI) 959
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.

Protocols

Product Specific Protocols
WB protocol for CD40L/CD154 antibody 66502-1-IgDownload protocol
IHC protocol for CD40L/CD154 antibody 66502-1-IgDownload protocol
FC protocol for CD40L/CD154 antibody 66502-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Pathol Res Pract

The potential role of follicular helper T cells in idiopathic multicentric Castleman disease with and without TAFRO syndrome.

Authors - Nozomu Kurose