CD7 Monoclonal antibody

CD7 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A4E6

Cat no : 60209-2-Ig

Synonyms

CD7, CD7 molecule, GP40, LEU 9, T cell antigen CD7, T cell leukemia antigen, T cell surface antigen Leu 9, Tp40, TP41



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, mouse thymus tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60209-2-Ig is CD7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD7 fusion protein Ag1805
Full Name CD7 molecule
Calculated molecular weight 240 aa, 25 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC009293
Gene symbol CD7
Gene ID (NCBI) 924
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD7 is a type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.

Protocols

Product Specific Protocols
WB protocol for CD7 antibody 60209-2-IgDownload protocol
IHC protocol for CD7 antibody 60209-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols