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  • Immunohistochemistry (IHC) staining of human placenta tissue using CD71 Recombinant antibody (81793-3-RR)

    IHC staining of human placenta using 81793-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81793-3-RR (CD71 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD71 Recombinant antibody (81793-3-RR)

    IHC staining of human placenta using 81793-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81793-3-RR (CD71 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD71 Recombinant monoclonal antibody (81793-3-RR)

    IHC staining of human tonsillitis using 81793-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81793-3-RR (CD71 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CD71 Recombinant antibody (81793-3-RR)

    IF Staining of K-562 using 81793-3-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-3-RR, Clone: 250738C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 81793-3-RR

    Affinity of 81793-3-RR

    Biolayer interferometry (BLl) kinetic assays of 81793-3-RR against Human CD71 were performed. The affinity constant is 31.5 pM.

CD71 Recombinant monoclonal antibody

CD71 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 81793-3-RR
Clone No.250738C5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

p90, sTfR, T9, TFR, TFR1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81793-3-RR targets CD71 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0339

Product name: Recombinant Human Transferrin R/CD71 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*HIS

Domain: 89-760 aa of

Sequence: CKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTSTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF

Predict reactive species
Full Name transferrin receptor (p90, CD71)
Calculated Molecular Weight85 kDa
Gene Symbol CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP02786
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
IF protocol for CD71 antibody 81793-3-RRDownload protocol
IHC protocol for CD71 antibody 81793-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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