CD86 Polyclonal antibody

CD86 Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, FC, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 13395-1-AP

Synonyms

Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 molecule, FUN 1, LAB72



Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells, RAW 264.7 cells
Positive IF detected inraji cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13395-1-AP targets CD86 in WB, IF, FC, Cell treatment, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD86 fusion protein Ag4063
Full Name CD86 molecule
Calculated molecular weight 329 aa, 38 kDa
Observed molecular weight 60-80 kDa
GenBank accession numberBC040261
Gene symbol CD86
Gene ID (NCBI) 942
RRIDAB_2074882
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. This antibody detects a band of approximately 65-70 kDa, consistent with glycosylated form of CD86 (PMID: 8592066, 21849678).

Protocols

Product Specific Protocols
WB protocol for CD86 antibody 13395-1-APDownload protocol
IF protocol for CD86 antibody 13395-1-APDownload protocol
FC protocol for CD86 antibody 13395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Adv Mater

Engineered Bacterial Outer Membrane Vesicles as Controllable Two-way Adaptors to Activate Macrophage Phagocytosis for Improved Tumor Immunotherapy

Authors - Qingqing Feng
mouseFC

Acta Pharm Sin B

Converting bacteria into autologous tumor vaccine via surface biomineralization of calcium carbonate for enhanced immunotherapy

Authors - Lina Guo
ratIF

Adv Healthc Mater

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.

Authors - Zhi Zheng
ratIF

Aging Cell

Donor BMSC-derived small extracellular vesicles relieve acute rejection post-renal allograft through transmitting Loc108349490 to dendritic cells.

Authors - Zhi-Gang Wang
mouseFC

Acta Biomater

Bacterial navigation for tumor targeting and photothermally-triggered bacterial ghost transformation for spatiotemporal drug release.

Authors - Songzhi Xie
mouseWB

ACS Appl Mater Interfaces

Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy.

Authors - Jiaojiao Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Martin (Verified Customer) (09-28-2022)

Antibody is working very well

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:300