Validation Data Gallery
|Positive WB detected in
|HepG2 cells, mouse placenta tissue, RAW 264.7 cells, HeLa cells, L02 cells, human placenta tissue, mouse liver tissue, rat liver tissue, THP-1 cells
|Positive IHC detected in
|human lung cancer tissue, human breast cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:6000
|IHC : 1:1000-1:4000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
20597-1-AP targets CD9 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, chicken, monkey, pig, Bat
|Host / Isotype
|Rabbit / IgG
|CD9 fusion protein Ag14546
|Calculated molecular weight
|228 aa, 25 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).
|Product Specific Protocols
|WB protocol for CD9 antibody 20597-1-AP
|IHC protocol for CD9 antibody 20597-1-AP
|IF protocol for CD9 antibody 20597-1-AP
|FC protocol for CD9 antibody 20597-1-AP
|Click here to view our Standard Protocols
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