CD9 Polyclonal antibody

CD9 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 20597-1-AP

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, DRAP 27, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29



Tested Applications

Positive WB detected inhuman placenta tissue, L02 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20597-1-AP is CD9 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD9 fusion protein Ag14546
Full Name CD9 molecule
Calculated molecular weight 228 aa, 25 kDa
Observed molecular weight 23-27 kDa
GenBank accession numberBC011988
Gene symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.

Protocols

Product Specific Protocols
WB protocol for CD9 antibody 20597-1-APDownload protocol
IHC protocol for CD9 antibody 20597-1-APDownload protocol
FC protocol for CD9 antibody 20597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Traffic

A toolbox for the immunomagnetic purification of signalling organelles.

Authors - Jürgen Fritsch
WB

Int J Biochem Cell Biol

Exosomes from hypoxia-treated human adipose-derived mesenchymal stem cells enhance angiogenesis through VEGF/VEGF-R.

Authors - Yudi Han
humanWB

Stem Cell Res Ther

Exosomes from glioma cells induce a tumor-like phenotype in mesenchymal stem cells by activating glycolysis.

Authors - Zhanjun Ma
humanWB

Mol Cancer

CD103-positive CSC exosome promotes EMT of clear cell renal cell carcinoma: role of remote MiR-19b-3p.

Authors - Lu Wang
human

Nanomedicine

Bronchial epithelial cell extracellular vesicles ameliorate epithelial-mesenchymal transition in COPD pathogenesis by alleviating M2 macrophage polarization.

Authors - Shengyang He
humanWB

Cancer Med

Identification and validation of circulating exosomes-based liquid biopsy for esophageal cancer.

Authors - An Zhao