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CDC25A Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 55031-1-AP

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Con: 80 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

CDC25A Antibody WB HepG2 cells 55031-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP(CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

CDC25A Antibody IHC human breast cancer tissue 55031-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 55031-1-AP( CDC25A Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CDC25A Antibody IHC human breast cancer tissue 55031-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 55031-1-AP( CDC25A Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CDC25A Antibody IF human breast cancer tissue 55031-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed human breast cancer tissue using 55031-1-AP (CDC25A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

CDC25A Antibody IP HeLa cells 55031-1-AP

IP Result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug.

Species specificity:
human, mouse
Positive WB detected in:
HepG2 cells, mouse liver tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human breast cancer tissue, human gliomas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human breast cancer tissue
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
cell division cycle 25 homolog A (S. pombe)
Calculated molecular weight:
59 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
NM_001789
Gene ID (NCBI):
993
Gene symbol
CDC25A
Synonyms
CDC25A, CDC25A2, M phase inducer phosphatase 1

Background

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.


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