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  • KD/KO Validated

CDC25A Polyclonal antibody

CDC25A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55031-1-AP

Synonyms

CDC25A, CDC25A2, M phase inducer phosphatase 1



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55031-1-AP targets CDC25A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cell division cycle 25 homolog A (S. pombe)
Calculated molecular weight 59 kDa
Observed molecular weight 60 kDa
GenBank accession numberNM_001789
Gene symbol CDC25A
Gene ID (NCBI) 993
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.

Protocols

Product Specific Protocols
WB protocol for CDC25A antibody 55031-1-APDownload protocol
IHC protocol for CDC25A antibody 55031-1-APDownload protocol
IF protocol for CDC25A antibody 55031-1-APDownload protocol
IP protocol for CDC25A antibody 55031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Transl Res

Decreased expression of miR-184 restrains the growth and invasion of endometrial carcinoma cells through CDC25A-dependent Notch signaling pathway.

Authors - Zhen Chen
  • KD Validated
humanWB

Cell Cycle

A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.

Authors - Yanan Wang
humanWB

Cell Cycle

PMID: 31599189

Authors - Yonglong Zhang
humanWB,IHC

Am J Transl Res

Inhibiting CREPT reduces the proliferation and migration of non-small cell lung cancer cells by down-regulating cell cycle related protein.

Authors - Tao Liu
mouseWB

Exp Cell Res

Cdc14a has a role in spermatogenesis, sperm maturation and male fertility.

Authors - Zongzhuang Wen
humanWB

Biomed Pharmacother

RNA sequencing analysis of the CAL-27 cell response to over-expressed ZNF750 gene revealed an extensive regulation on cell cycle.

Authors - Xianbin Liu