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CDC25A Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 55031-1-AP

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Con: 80 μg/150 μl

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Tested Applications
Applications:
ELISA, WB, IP, IHC
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Images See all

55031-1-AP;HepG2 cells

HepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP(CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

55031-1-AP;HeLa cells

IP Result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug.

55031-1-AP;human breast cancer tissue

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 55031-1-AP( CDC25A Antibody) at dilution of 1:50 (under 10x lens)

55031-1-AP;human breast cancer tissue

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 55031-1-AP( CDC25A Antibody) at dilution of 1:50 (under 40x lens)

Species specificity:
human, mouse
Positive WB detected in:
HepG2 cells, mouse liver tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human breast cancer tissue, human breast cancer tissue, human gliomas tissue
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
cell division cycle 25 homolog A (S. pombe)
Calculated molecular weight:
59 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
NM_001789
Gene ID (NCBI):
993
Gene symbol
CDC25A
Synonyms
CDC25A, CDC25A2, M phase inducer phosphatase 1
Background

CDC25A blongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.


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