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Tested Applications
| Positive WB detected in | HepG2 cells, mouse liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 38 publications below |
| IHC | See 7 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
Product Information
55031-1-AP targets CDC25A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | cell division cycle 25 homolog A (S. pombe) |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | NM_001789 |
| Gene Symbol | CDC25A |
| Gene ID (NCBI) | 993 |
| RRID | AB_2881258 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30304 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC25A antibody 55031-1-AP | Download protocol |
| IHC protocol for CDC25A antibody 55031-1-AP | Download protocol |
| IF protocol for CDC25A antibody 55031-1-AP | Download protocol |
| IP protocol for CDC25A antibody 55031-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Immunol A critical role of N4-acetylation of cytidine in mRNA by NAT10 in T cell expansion and antiviral immunity | ||
Nat Commun Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance. | ||
Nat Commun The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells. | ||
Pharmacol Res A Modified Nucleoside O6-methyl-2'-deoxyguanosine-5'-Triphosphate Exhibits Anti-Glioblastoma Activity in a Caspase-Independent Manner | ||
Cell Death Dis ZNF652 exerts a tumor suppressor role in lung cancer by transcriptionally downregulating cyclin D3 |
