CDH23 Polyclonal antibody

CDH23 Polyclonal Antibody for WB, ELISA

Cat No. 13496-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

CAD23, Cadherin 23, cadherin like 23, CDH23, DFNB12, KIAA1774, KIAA1812, Otocadherin, USH1D

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of A431 using 13496-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 13496-1-AP (CDH23 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Y79 using 13496-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 13496-1-AP (CDH23 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for CDH23 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

A431 cells, Y79 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13496-1-AP targets CDH23 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4322 Product name: Recombinant human CDH23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC032581 Sequence: MGRHVATSCHVAWLLVLISGCWGQVNRLPFFTNHFFDTYLLISEDTPVGSSVTQLLAQDMDNDPLVFGVSGEEASRFFAVEPDTGVVWLRQPLDRETKSEFTVEFSVSDHQGVITRKVNIQVGDVNDNAPTFHNQPYSVRIPENTPVGTPIFIVNATDPDLGAGGSVLYSFQPPSQFFAIDSARGIVTVIRELDYETTQAYQLTVNATDQDKTRPLSTLANLAIIITDVQDMDPIFINLPYSTNIYEHSPPGTTVRIITAIDQDKGRPRGIGYTIVSGNTNSIFALDYISGVLTLNGLLDRENPLYSHGFILTVKGTELNDDRTPSDATVTTTFNILVIDINDNAPEFNSSE Predict reactive species
Full Name	cadherin-like 23
Calculated Molecular Weight	3354 aa, 369 kDa
Observed Molecular Weight	59 kDa
GenBank Accession Number	BC032581
Gene Symbol	CDH23
Gene ID (NCBI)	64072
RRID	AB_10667006
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H251
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CDH23 antibody 13496-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The CDH23 polyclonal antibody (Cat# 13496-1-AP) has 1 total citation. The cited publication appeared in Human Molecular Genetics and investigated the impact of Usher syndrome on olfaction, placing the research in the fields of human genetics, inherited sensory disorders, and olfactory biology. The antibody was applied in Western blot and immunofluorescence using mouse samples.