Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25235-1-AP targets CEP110 in WB, IF/ICC, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18907 Product name: Recombinant human CEP110 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1996-2325 aa of BC137284 Sequence: VLAAEERVRTLQEEERWCESLEKTLSQTKRQLSEREQQLVEKSGELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLKEALKIQRSQLEKNLLEQKQENSCIQKEMATIELVAQDNHERARRLMKELNQMQYEYTELKKQMANQKDLERRQMEISDAMRTLKSEVKDEIRTSLKNLNQFLPELPADLEAILERNENLEGELESLKENLPFTMNEGPFEEKLNFSQVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLIKGKRQTEGTLHSLRRQVDALGELVTSTSADSASSPSLSQLESSLTEDSQLGQNQEKNASAR Predict reactive species |
| Full Name | centrosomal protein 110kDa |
| Calculated Molecular Weight | 2325 aa, 269 kDa |
| Observed Molecular Weight | 270 kDa |
| GenBank Accession Number | BC137284 |
| Gene Symbol | CEP110 |
| Gene ID (NCBI) | 11064 |
| RRID | AB_2879979 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z7A1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP110 antibody 25235-1-AP | Download protocol |
| WB protocol for CEP110 antibody 25235-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One user rated this antibody 4 out of 5 stars. They used it at a 1:100 dilution for immunofluorescence on mouse NIH3T3 cells with methanol fixation. The reviewer reported clear localization of CEP110 at the centrosome, co-stained with gamma-Tubulin as a centrosome marker and DAPI for nuclear counterstaining. Overall, the feedback is positive, confirming the antibody performs well for IF applications in mouse cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jay (Verified Customer) (10-28-2019) | Methanol fixation.The first IF result showing that cep110 localizes at centrosome in mouse NIH3T3 cell line(Red;Cep110, Green;gamma-Tubulin for centrosome marker, Blue;DAPI for nucleus.)
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