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  • KD/KO Validated

CHCHD6 Antibody

CHCHD6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20639-1-AP

Synonyms

CHCHD6, CHCM1, MIC25



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 20639-1-AP immunogen is Fusion Protein CHCHD6 protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHCHD6 fusion protein Ag14700
Full Name coiled-coil-helix-coiled-coil-helix domain containing 6
Calculated molecular weight 235aa,26 kDa
Observed molecular weight 26-29 kDa
GenBank accession number BC006123
Gene symbol CHCHD6
Gene ID (NCBI) 84303
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHCHD6 antibody 20639-1-APDownload protocol
IHC protocol for CHCHD6 antibody 20639-1-APDownload protocol
IP protocol for CHCHD6 antibody 20639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.

Authors - Chengli Ding
  • KO Validated
mouseWB

Cell Death Differ

Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Authors - H Li
  • KD Validated
humanWB

Elife

QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease.

Authors - Virginia Guarani
humanWB

EMBO Rep

Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.

Authors - Arun Kumar Kondadi
humanWB

Elife

QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.

Authors - Virginia Guarani

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jingwen (Verified Customer) (01-28-2020)

This antibody worked very well when I performed western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cancer tissue
No image submitted with review