|Positive WB detected in||HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The 20639-1-AP immunogen is Fusion Protein CHCHD6 protein expressed in E. coli.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHCHD6 fusion protein Ag14700|
|Full Name||coiled-coil-helix-coiled-coil-helix domain containing 6|
|Calculated molecular weight||235aa,26 kDa|
|Observed molecular weight||26-29 kDa|
|GenBank accession number||BC006123|
|Gene ID (NCBI)||84303|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.
Cell Death Differ
Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.
QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease.
Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.
QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jingwen (Verified Customer) (01-28-2020)
This antibody worked very well when I performed western blot.