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  • KD/KO Validated

CHCHD6 Polyclonal antibody

CHCHD6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20639-1-AP

Synonyms

CHCHD6, CHCM1, MIC25



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20639-1-AP is CHCHD6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHCHD6 fusion protein Ag14700
Full Name coiled-coil-helix-coiled-coil-helix domain containing 6
Calculated molecular weight 235 aa, 26 kDa
Observed molecular weight 26-29 kDa
GenBank accession numberBC006123
Gene symbol CHCHD6
Gene ID (NCBI) 84303
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHCHD6 antibody 20639-1-APDownload protocol
IHC protocol for CHCHD6 antibody 20639-1-APDownload protocol
IP protocol for CHCHD6 antibody 20639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Life Sci Alliance

MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes.

Authors - Ruchika Anand
humanWB

Sci Rep

Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.

Authors - Chengli Ding
  • KO Validated
mouseWB

Cell Death Differ

Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Authors - H Li
  • KD Validated
humanWB

Elife

QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease.

Authors - Virginia Guarani
humanWB

EMBO Rep

Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.

Authors - Arun Kumar Kondadi
humanWB

Elife

QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.

Authors - Virginia Guarani

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

SITING (Verified Customer) (07-13-2020)

very clear band in our wester blot expose result

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MOUSE HEART
FH

Jingwen (Verified Customer) (01-28-2020)

This antibody worked very well when I performed western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cancer tissue