|Positive WB detected in||HeLa cells, HEK-293 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14173-1-AP targets CHD4 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHD4 fusion protein Ag5372|
|Full Name||chromodomain helicase DNA binding protein 4|
|Calculated molecular weight||1937 aa, 221 kDa|
|Observed molecular weight||260 kDa|
|GenBank accession number||BC038596|
|Gene ID (NCBI)||1108|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calculated molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.
|Product Specific Protocols|
|WB protocol for CHD4 antibody 14173-1-AP||Download protocol|
|IHC protocol for CHD4 antibody 14173-1-AP||Download protocol|
|IF protocol for CHD4 antibody 14173-1-AP||Download protocol|
|IP protocol for CHD4 antibody 14173-1-AP||Download protocol|
|Click here to view our Standard Protocols|
CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma.
Functional Genomic Analysis of a RUNX3 Polymorphism Associated with Ankylosing Spondylitis.
Runx1 repression by histone deacetylation is critical for Setbp1-induced mouse myeloid leukemia development.
ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression.